《植物生理学报》 2016, 52(4): 471-478

植物生长物质对白英毛状根生长及薯蓣皂苷元含量的影响

董瑜¹, 陈宇¹, 张楷燕¹, 孙一铭¹, 张来², 孙敏^1,*

¹西南大学生命科学学院, 三峡库区生态环境教育部重点实验室, 重庆400715; ²安顺学院, 贵州省教育厅功能材料与资源化学特色重点实验室, 贵州安顺561000

通信作者：孙敏；E-mail: jwcsm@swu.edu.cn

摘　要：

利用含pRiA4质粒的农杆菌C58C1浸染白英叶片获得能在无植物生长物质培养基上自主生长的毛状根单克隆系, 且毛状根中薯蓣皂苷元含量高于野生型白英植株。在液体培养基中添加不同浓度的6-苄氨基腺嘌呤(6-BA)、萘乙酸(NAA)或6-BA与NAA的结合处理白英毛状根, 观察其对毛状根生长的影响, 并利用高效液相色谱法(HPLC)测定毛状根中薯蓣皂苷元含量。结果表明, 6-BA抑制白英毛状根的生长, 且随6-BA浓度增加, 抑制作用越明显, 6-BA能增加白英毛状根每克干物质中薯蓣皂苷元的含量, 但降低培养瓶中毛状根薯蓣皂苷元总量。NAA对毛状根的生长有促进作用, 其中, 经0.5 mg•L^-1NAA处理的毛状根在培养30 d时干重达到同期对照的1.76倍。虽然NAA降低每克干物质中薯蓣皂苷元含量, 但提高培养瓶中毛状根薯蓣皂苷元总量, 薯蓣皂苷元总量最高可达0.97 mg (DW)•瓶^-1, 是对照整个培养过程中最大值的1.37倍。6-BA与0.5 mg•L^-1 NAA的结合抑制白英毛状根的生长, 同时降低每克干物质中薯蓣皂苷元的含量。

关键词：白英; 毛状根; 植物生长物质; 薯蓣皂苷元; HPLC

收稿：2016-02-25   修定：2016-03-22

资助：中央高校基本科研业务费专项资金项目(XDJK2012C088)和贵州省功能材料与资源化学特色重点实验室开放基金项目。

Effects of plant growth substance on growth and diosgenin content of Solanum lyratum hairy roots

DONG Yu¹, CHEN Yu¹, ZHANG Kai-Yan¹, SUN Yi-Ming¹, ZHANG Lai², SUN Min^1,*

¹Key Laboratory of Eco-Environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China; ²Special and Key Laboratory of Functional Materials and Resource Chemistry of Guizhou Provincial Education Department, Anshun University, Anshun, Guizhou 561000, China

Corresponding author: SUN Min; E-mail: jwcsm@swu.edu.cn

Abstract:

Hairy roots of Solanum lyratum could be obtain by infecting leaf explants with Agrobacterium rhizogenes C58C1 harboring pRiA4 plasmid. The hairy roots could grow rapidly on medium without plant growth substance and contained more diosgenin than the wild S. lyratum. In order to study the effects of plant growth substance on the growth and diosgenin contents of S. lyratum hairy roots, we cultured the hairy roots with different concentrations of 6-benzylaminopurine (6-BA), α-naphthaleneacetic (NAA) alone or 6-BA in combination with NAA. The diosgenin of hairy roots were examined by high performance liquid chromatography (HPLC). The results showed that 6-BA inhibited the growth, and increased the hairy roots per gram of dry matter contents of diosgenin. But 6-BA decreased the content of total diosgenin. Furthermore, the inhibition was increased with the concentration of 6-BA. NAA promoted the growth and increased the content of total diosgenin. The hairy roots cultured with 0.5 mg•L^-1 NAA after 30 d, the weight was 1.76 times of control, and the content of total diosgenin reached 0.97 mg (DW)•flask^-1, it’s 1.37 times of maximum value of control. Different concentrations of 6-BA in combination with 0.5 mg•L^-1 NAA could inhibit the growth of hairy roots and decrease the content of total diosgenin.

Key words: Solanum lyratum; hairy roots; plant growth substance; diosgenin; HPLC